The study on the isolation and evaluation of bioactivities of endophytic fungi from. Endophytic fungi are promising and plentiful sources of an extensive range of beneficial natural products with varied chemical structures, higher bio diversities and numerous fascinating bioactivities 10. They have attracted researchers due to their importance in ecology calcul et al. Endophytic fungi have been shown to produce allelopathic compounds that poison neighboring plants. The present study examined anti bacterial and antioxidant potential of endophytic fungi isolated from two mangrove plants.
Biological activities of extracts from endophytic fungi. Extracellular enzymatic activities of endophytic fungi. Diversity and antimicrobial and plantgrowthpromoting. Samuelse, nancy robbinsa, edward allen herrea, asmithsonian tropical research institute, apartado postal 084303092, balboa, ancon, panama b department of plant biology and pathology, rutgers university. Isolation, screening for bioactivities and identification. Study of antidiabetic activities of endophytic fungi. Isolation and identification of endophytic fungi from. Antimicrobial activity of endophytic fungi isolated from. Isolation, characterization, and bioactivity of endophytic. Alternariatenuissima, colletotrichum truncatum, and alternaria sp.
Endophytic fungi were identified by using morphological and molecular biological methods. Antibacterial and antioxidant activities of endophytic. Antibacterial and antioxidant potential of endophytic fungi. Pdf isolation and identification of endophytic fungi from. This study elucidates the mechanisms employed by endophytes in protecting the plant from diseases and different bioactivities of importance to humans with a focus on endophytic bacteria and fungi. Pdf diversity and bioactivities of fungal endophytes. It also possesses diverse pharmacological effects such as antiinflammatory annegowda et al. Apr 07, 2018 the term endophytes includes a family of microorganisms that grow intraandor intercellularly in the tissues of higher plants without any symptoms on the plants in which they reside. This was the first study to evaluate their cytotoxic and antitumour. Pdf bioactivity and genetic of endophytic fungi in rhododendron. In the present investigation ten medicinal plants were analyzed for the presence of endophytic fungi. Diversity and bioactivities of fungal endophytes from distylium.
Endophytic fungi may be from hypocreales and xylariales of the sordariomycetes pyrenomycetes class or from the class of loculoascomycetes. The lichens investigated were two foliose lichens, pseudocyphellaria sp. Bioactivities of the metabolites from endophytic fungi associated with ficus elastica. Endophytic fungi are an important, yet relatively unstudied group of microbial plant symbionts.
Most endophytes known are bacteria or fungi, although there are also some endophytic algae and oomycetes. Antimicrobial activity of endophytic fungi from leaves and barks of. Endophytes are distinct from mycorrhizal fungi or rhizosphere microbes in that they live entirely within the plant. Mar, 2020 endophytic fungi isolated from medicinal plants are important for production of antibiotics. Fungal endophytes were isolated from healthy plant parts such as.
Samif01 and its bioactivities jingfeng loua, ruiting yu a. The toxicity of endophytic fungi was evaluated in vitro using the brine shrimp shortterm bioassay, a useful tool to test plant extract bioactivity that correlates with cytotoxic and antitumour properties 28. In exchange for carbohydrate energy resources, the fungus provides benefits to the plant which can include increased water or nutrient uptake and protection from. As endophytes living inside the plants increases the resistance capacity of the plant towards undesirable microbial attacks and stresses. Bioactive secondary metabolities from endophytic aspergillus. This study was focused to isolate and identify culturable endophytic fungi from foliar parts. Plant endophytic fungi are defined as fungi that live asymptomatically within plant tissues. Bioactivities and future perspectives of chaetoglobosins.
One group of fungal endophytes are the arbuscular mycorrhizal fungi. Endophytic fungi live asymptomatically, and sometimes systemically, within plant tissues carroll, 1988, 1991. The fungi used in this study are the endophytic fungi isolated from different medicinal plants, azadirachta indica, citrus limon, gossypium hirsutum, magnolia champaca, datura stramonium, piper betle, phyllanthus emblica collected from the in k. Some of the isolates of endophytic fungi exhibited significant inhibitory activity on selected test organisms. Bioactive secondary metabolites from endophytic aspergillus scielo. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. An endophyte is an endosymbiont, often a bacterium or fungus, that lives within a plant for at least part of its life cycle without causing apparent disease.
Many endophytes are in the phyla basidiomycota and ascomycota. Pdf isolation and identification of endophytic fungi from actinidia. A total of 81 thai medicinal plant species collected from forests were examined for the presence of endophytic fungi with biological activity. The secondary metabolites like alkaloids, indole derivatives, steroids, terpenoids, quinones, flavonoids. Diversity, bioactivities, and metabolic potentials of. Five different taxa were represented by 11 isolates, and six isolates were grouped. Endophytic fungi occur within plant tissues such as leaves and stems of healthy plants without producing any apparent infections and symptoms. The extracts of 12 endophytes exhibited inhibitory effects on collageninduced platelet aggregation with ic50 values of 19. Endophytes usually inhabit aboveground plant tissues leaves, stems, bark, petioles and reproductive structures, which distinguishes. Antibacterial and antioxidant activities of endophytic fungi extracts of. Five different taxa were represented by 11 isolates, and six isolates were. Antimicrobial and cytotoxic activities of endophytic. Aquatic endophytic fungi pteridophyte statistical analysis.
Endophytic fungi are a group of fungi that colonize living and internal tissues of plants without causing any immediate, overt negative effects. Endophytic fungi are ubiquitously distributed in orchids and have a great impact on the host plant. Perhaps this reduces competition in favor of plant species than can host said endophytes. To date, around 100 chaetoglobosins and their analogues have been isolated and identified over the years from a variety of fungi, mainly from the fungus chaetomium globosum. Of the seven endophytic fungi isolated, four were found to exhibit anti bacterial activity. Alternariol 9methyl ether was active against bacteria. Pdf the first report of embellisia abandance as an. Dreyfuss and chapela 1994 estimated that endophytic fungi, alone could be. Studies had shown previously to protect watermelon citrullus lanatus and cucumber cucumis sativus seedlings from anthracnose disease elicited by wildtype c. Identification and bioactivities of two endophytic fungi. Antimicrobial and cytotoxic activities of endophytic fungus. Endophytic fungi isolated from medicinal plants are important for production of antibiotics. Endophytic fungi from the chinese medicinal plant actinidia macrosperma were isolated and identified for the first time. Antibacterial and antioxidant potential of endophytic.
Endophytic fungi of the genera acremonium, beauveria, cladosporium, clonostachys, paecilomyces. Diversity and bioactive potential of culturable fungal endophytes of. Endophytic fungi as biocontrol agents of theobroma cacao pathogens luis c. Alternariol 9methyl ether from the endophytic fungus. Endophytic fungi from medicinal plants wiyakrutta et al. Evaluation of antimicrobial activity of endophytic fungi. Cytotoxic metabolites of endophytic fungi from mangrove plants. They can produce secondary metabolites with diverse structures and activities. In this study, four benzopyranones were isolated from the ethyl acetate extract of hyalodendriella sp. Colonization frequency, endophytic infection rate and bioactivities of microbes of desert medicinal plants. Pdf biological activities of extracts from endophytic fungi isolated. Sixtyfive crude extracts from 51 selected endophytic fungi isolated from garcinia species were tested for various bioactivities. Ponipodef12, and identified as palmariol b 1, 4hydroxymellein 2, alternariol 9methyl ether 3, and botrallin 4 by means of physicochemical and. Unlike mycorrhizal fungi that colonize plant roots and.
Endophytic definition of endophytic by the free dictionary. Plant use of endophytic fungi in defense occurs when endophytic fungi, which live symbiotically with the majority of plants by entering their cells, often fungus or bacteruim, are utilized as an indirect defense against herbivores. Areas receiving lots of plant litter containing endophytic fungi produced fewer plants but these plants grew larger than areas without endophytic fungi litter. Ponipodef12 was isolated from the hybrid neva of populus deltoides marsh.
Copyright mushroom research foundation 2016 diversity of. Pdf eightyseven endophytic fungi were isolated from asymptomatic leaf tissues of rhododendron tomentosum. Copyright mushroom research foundation 2016 diversity. Isolation, screening for bioactivities and identification of.
A total of 25 fungal species were isolated from healthy leaves and identified based on the morphology of. Screening of endophytic fungi with antiphytopathogen. It is obvious that endophytic fungi have now been identified as a store house of secondary. The tree was derived by neighborjoining methods analysis of its15. Pdf isolation and identification of endophytic fungi. Eighty per cent of the fungal extracts from fermentation broths and mycelia displayed bioactivities.
In summary, the results of this study further our microecological understanding of endophytic fungi in their a. Study of antidiabetic activities of endophytic fungi isolated. Vegab and soroush parsaa aentomology, international center for tropical agriculture ciat, cali, colombia. Isolation of endophytic fungi and screening of huperzine a. However, even with the accumulating evidence that endophytic fungi can reduce pathogen damage in grasses and. It was hypothesized that endophytic fungi from this medicinal plant possess potentials as bioactive compound producers. Diversity and bioactivities of fungal endophytes from distylium chinense, a rare waterlogging. Antibacterial and antioxidant activities of endophytic fungi. Endolichenic fungal strains, fungi that live asymptomatically in the lichen thallus, much the same way as endophytic fungi live within. Gao 1school of plant protection, anhui agricultural university, hefei, china 2school of life science, anhui agricultural university, hefei, china. Frontiers elucidating mechanisms of endophytes used in.
Fungal endophytes of an aquatic weed marsilea minuta linn. Endophytic fungi produce secondary metabolites similar to the host plant. Pdf bioactivity assessment of endophytic fungi associated with. Diversity and bioactivities of fungal endophytes from. To better utilize this resource, biodiversity and bioactivity of endophytic fungi isolated from a. In recent years, an increasing number of bisnaphtho. A total of 153 endophytic fungi were isolated from 94 fresh plants and purified from 1974 tissue segments stems, leaves, and roots of. Pdf the present study attempted to evaluate the antimicrobial and antioxidant activity of fungal endophytes associated with centella asiatica and.
A novel method to scale up fungal endophyte isolations. Till now, meagre work has been done related to isolation and bioactivities of endophytic fungi. The endophytic extracts from 19 endophytes, isolated from species of taiwanese plants, were evaluated for biological activity, including cytotoxicity, antiplatelet aggregation, and antiinflammatory activity. Bioactivity evaluation of fungal endophytes as mentioned above, one of the main purposes of this study was to identify endophytic fungi that could be cultured and applied to develop their potentially beneficial properties for plants and humans. This was the first study to evaluate their cytotoxic and antitumour activities against brine shrimp and five types of tumour cells, respectively. Here, in an attempt to explore endophytic fungi from healthy foliar tissues of the plant p. Endophytic fungi as biocontrol agents of theobroma cacao. Pdf endophytic fungi from the chinese medicinal plant actinidia macrosperma. Diversity of endophytic fungi from the ornamental plant adenium obesum. Endolichenic fungal strains, fungi that live asymptomatically in the lichen thallus, much the same way as endophytic fungi live within healthy plant tissues, were isolated from three abundant lichen species, pseudocyphellaria sp. Most of the endophytes were obtained from the leaves and very few from the stems. The diversity of endophytic fungi in the medicinal orchid dendrobium loddigesii rolfe was investigated and their bioactivities in microbe and plant growth were explored here.
The reoccurrence rate of the endophytic fungi was 1. Isolation and identification of endophytic fungi from various. Endophytes are ubiquitous and have been found in all species of plants studied to date. Isolation and identification of endophytic fungi from actinidia macrosperma and investigation of their bioactivities. Endophytic fungal diversity in medicinal plants of western. Pdf sixtyfive crude extracts from 51 selected endophytic fungi isolated from garcinia species were tested for various bioactivities.
Chaetoglobosins belonging to cytochalasan alkaloids represent a large class of fungal secondary metabolites. Fungi from actinidia macrosperma and investigation of their bioactivities. Experiments were conducted to isolate endophytic fungi from healthy medicinal plants as well as detecting any reoccurrence of any particular endophytic fungi predominantly in selected local medicinal plants. Bioactivities of endophytic penicillia from cupressaceae article pdf available in crop protection 24. Cinnamomum species are widely used in herbal therapy in treating colds, sinusitis, bronchitis and fungal infection kharwar et al. Endophytic fungi isolated from different parts of plants have a more beneficial effect and therefore endophytes can be used in various applications. Samif01 residing in the roots of salvia miltiorrhiza bunge. Mangrove endophytic fungi are the second largest ecological group of marine fungi and are potential sources for isolation of new bioactive compounds kalaiselvam, 2015. Pdf bioactivities of endophytic penicillia from cupressaceae.
Debregeasia salicifolia is a plant of medicinal importance, and no report exists regarding isolation of endophytic fungi from it. Frontiers endophytic fungialternative sources of cytotoxic. The endophytic fungi were subcultured on pda medium. Plant use of endophytic fungi in defense wikipedia. The aim of this study was to investigate the nature and bioactivities of endolichenic fungi in three abundant lichens, pseudocyphellaria sp. The present study was undertaken to investigate the nature and bioactivities of endolichenic fungi in three abundant lichens in the lower elevation of hakgala montane forest in sri lanka. Bioactivity assessment of endophytic fungi associated with centella asiatica and murraya. The term endophytes includes a family of microorganisms that grow intraandor intercellularly in the tissues of higher plants without any symptoms on the plants in which they reside. Bioprospecting of novel and bioactive metabolites from endophytic. Studies have found that chaetoglobosins possess a broad range of biological activities, including antitumor. Endophytes constitute an important component of microbial diversity, and in the present investigation, seven plant species with rich ethnobotanical uses representing six families were analyzed for the presence of endophytic fungi from their natural habitats during monsoon mayjune and winter novemberdecember seasons of 2007.
291 1272 1606 634 1443 1598 133 532 315 1355 455 953 1353 2 487 463 539 1109 969 224 1594 638 1558 959 719 222 1070 1086 513 1513 1048 744 629 1149 1448 337 670 973 522 509 184 200 365 1161 18 515 726